Milking of cows on-farm is usually done twice per day in milking parlors. Modern milking units cn be applied automatically in robotic milkers, or manually in herring-bone style or rotary milking parlor configurations. The milking unit simulates the suckling action by pulsating a rubber inflation to massage the teat and remove the milk. Vacuum is applied to create the pulsating opening and closing of the rubber inflation, but the vacuum is not applied directly to the teat. Milk is withdrawn at 37°C and must be immediately cooled to < 4°C.